Detection of Human Papillomavirus Subtype 16 in Esophageal Squamous Cells Carcinoma in Sudanese Patients

Oesophageal cancer is the sixth most common among males and ninth females worldwide. The purpose of this study to detect association Human papilloma virus subtype 16 esophageal squamous cell carcinoma in Khartoum state Hospitals by polymerase chain reaction method. A retrospective descriptive archival formalin fixed paraffin embedded tissue from Esophageal Squamous cells specimens acquired at Omdurman Teaching hospital, Alribat, IbnSina Hospital Military hospital. 50 were used HPV-16 DNA Extraction method (PCR). PCR was performed on formalin-fixed, paraffin-embedded samples patients with ESCCS. detection role HPV-16. SPSS analyze data, histological grade tumors determined. In 18 % cases, presence positive ages above 40 years old (54.2%). Females predominantly affected cellscarcinoma (22.6%). differentiation observed high rate human type found poorly differentiated (20%). frequency -16 statistically insignificant associated gender, age differentiation. (P value < 0.05).